DOIONLINE

DOIONLINE NO - IJEEDC-IRAJ-DOIONLINE-17949

Publish In
International Journal of Electrical, Electronics and Data Communication (IJEEDC)-IJEEDC
Journal Home
Volume Issue
Issue
Volume-9,Issue-4  ( Apr, 2021 )
Paper Title
A Novel Method for Peak Calling of Chip-SEQ Data Based on Scale Space Filtering to Combat Against Random Variations
Author Name
Jahanzeb Saqib, Unjoo Lee
Affilition
Research Assistant, Department of Electronic Engineering, Hallym University, Chuncheon, Korea Associate Professor, Department of Electronic Engineering, Hallym University, Chuncheon, Korea
Pages
22-27
Abstract
This paper proposes a novel algorithm for peak calling in chromatin immune precipitation sequencing (ChIP-Seq) data using scale-space-filtering. In this method, Gaussian convolution is applied to expand the given signal over a continuum of sizes. The generated scale space image is explored just like a tree by using its qualitative structure having concise but complete description of observation at all possible scales. It is of great practical importance and quite challenging to understand genome-wide DNA sequencing for the identification of transcription factor binding sites in large-scale ChIP-Seq data. This method explores not only enriched peak regions effectively but also low-density background regions as well. Computer simulations reveal that the proposed methodology has a great potential to produce good results by accurately estimating the average fragment length and threshold level. Keywords- Chromatin Immune Precipitation Sequencing, Gaussian Convolution, Scale-Space-Filtering, Transcription Factor Binding Sites.
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