DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLNE-14751

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-6, Issue-4, Spl. Iss-2  ( Dec, 2018 )
Paper Title
Toxicity Assessment of Nanoemulsion Containing the Essential Oil of Rosmarinus officinalis L. in Zebrafish
Author Name
Raphaelle Sousa Borges, Brenda Lorena Sanchez-Ortiz, Arlindo Cesar Matias Pereira Filho, Hady Keita, Nayara Costa De Melo, Jose Carlos Tavares Carvalho
Affilition
Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e Saúde, Universidade Federal do Amapá (UNIFAP), Macapá, AP, Brasil; 4Division de Pos-Grado, Instituto de Investigación sobre la Salud Publica, Ciudad Universitaria, Universidad de la Sierra Sur, Oaxaca, Mexico
Pages
48-51
Abstract
The essential oil of Rosmarinus officinal is (EORO) has been used for medicinal purposes around the world, which encouraged research toward the elucidation of its biological activities. Currently, a methodology was developed to encapsulate it in the form of a nanoemulsion (NEORO), which managed to improve the pharmacological activities of EORO using lower doses. However, no toxicological evaluation were performed, and hence, this study aimed to evaluate the toxicity of NEORO in zebrafish (Danio rerio), obtaining the LD50 value and analyzing histopathologic alterations of liver, kidney, and intestine. Zebrafishes were treated with crescent doses of NEORO (1, 2, 3, 4 and 5 mg/kg), and the LD50 value was 2.4 mg/kg. Histologically, kidney and intestine were more altered, while the liver remained relatively normal, despite being accountable for a significant part of toxic compounds metabolism in zebrafish. Index Terms - Essential Oil, Nanoemulsion, Rosmarinus Officinal is, Zebrafish.
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