DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLNE-14287

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-6, Issue-4  ( Oct, 2018 )
Paper Title
Investigation of Polymorphism and Mutation in the Exon1 Region of MBL2 Gene at Patients with COPD
Author Name
Negar Aliyari, Bahram Golestani Eimani, Reza Talebi
Affilition
Department of Biology, Islamic Azad University, Urmia Branch, Urmia, Iran. Department of Biology, Faculty of Sciences
Pages
109-112
Abstract
Introduction and objectives: Obstructive pulmonary disease (COPD) is characterized by chronic airway limitation. The most common causes of COPD are smoking and respiratory infections. Mannose-binding lectin (MBL) belongs to the family of collectins and plays a role in the complement activation of the innate immunity through the lectin pathway. Materials and Methods: In this study, following DNA extraction in 59 patients with COPD, PCR was used to amplify the exon 1 of MBL2 gene. The PCR products were later sequenced and BLAST search was performed on them. The results were analyzed using SPSS. Results: The results of nonparametric statistics showed that there was a significant relationship only between nucleotides 304 deletion mutation (T) of the gene MBL2 exon 1 and there was not a significant correlation between the response to treatment and remaining polymorphisms and mutations and in the studied subjects. Keywords – Mannose Binding Lectin (MBL), Chronic Obstructive Pulmonary Disease (COPD), Polymerase Chain Reaction (PCR), Polymorphism
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