Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
Journal Home Volume Issue |
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Issue |
Volume-6, Issue-4 ( Oct, 2018 ) | |||||||||
Paper Title |
Investigation of Polymorphism and Mutation in the Exon1 Region of MBL2 Gene at Patients with COPD | |||||||||
Author Name |
Negar Aliyari, Bahram Golestani Eimani, Reza Talebi | |||||||||
Affilition |
Department of Biology, Islamic Azad University, Urmia Branch, Urmia, Iran. Department of Biology, Faculty of Sciences | |||||||||
Pages |
109-112 | |||||||||
Abstract |
Introduction and objectives: Obstructive pulmonary disease (COPD) is characterized by chronic airway limitation. The most common causes of COPD are smoking and respiratory infections. Mannose-binding lectin (MBL) belongs to the family of collectins and plays a role in the complement activation of the innate immunity through the lectin pathway. Materials and Methods: In this study, following DNA extraction in 59 patients with COPD, PCR was used to amplify the exon 1 of MBL2 gene. The PCR products were later sequenced and BLAST search was performed on them. The results were analyzed using SPSS. Results: The results of nonparametric statistics showed that there was a significant relationship only between nucleotides 304 deletion mutation (T) of the gene MBL2 exon 1 and there was not a significant correlation between the response to treatment and remaining polymorphisms and mutations and in the studied subjects. Keywords – Mannose Binding Lectin (MBL), Chronic Obstructive Pulmonary Disease (COPD), Polymerase Chain Reaction (PCR), Polymorphism | |||||||||
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