Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
Journal Home Volume Issue |
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Issue |
Volume-6, Issue-3, Spl. Iss-2 ( Sep, 2018 ) | |||||||||
Paper Title |
Effect of Horseradish Peroxidase for the Control of Hydrogen Peroxide Mediated Signal Generation through the Growth of Gold Nanoparticles | |||||||||
Author Name |
Yufei Cheng, Chen Situ | |||||||||
Affilition |
Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, UK | |||||||||
Pages |
67-70 | |||||||||
Abstract |
In classical colorimetric immunoassays the signal for detection is generated by a bio-catalytic conversion of a molecule (antigen or antibody) by an enzyme which acts as an amplifier. However, a sensitive and reliable immunoassay requires high selectivity and affinity of antibodies to their corresponding antigens that forms the principle of bio-recognition. If the presence of analyte is at very low levels, immunoassay is unable to detect them and as such necessitates the use of more sensitive methods. Nanoparticles (e.g. gold or silver colloids) represent a new signal generation mechanism for bio-sensing giving the required improvements in signal production. A catalytic approach involving hydrogen peroxide and gold nanoparticles has recently been demonstrated and has the potential to be applied to a wide variety of food safety targets such as chemical and microbiological contaminants, veterinary drug residues, and protein biomarkers. Keywords - Bio-recognition, Nanoparticle, Plasmonic, Contaminant. | |||||||||
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