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International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
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Volume Issue
Volume-4, Issue-4  ( Oct, 2016 )
Paper Title
Studies in Salt Tolerant Alkaline Protease of Bacterium Isolated From Konkan Coast
Author Name
M. Sambhare, Y. Shouche, P. Harikumar, M. Kamat
Mithibai College of Arts, Chauhan Institute of Science and Amrutben Jivanlal College of Commerce and Economics, Vile Parle (W.), Mumbai-400 056 2Microbial Culture Collection (MCC), National Center for Cell Science (NCCS), NCCS Complex, Pune University Campus , Ganeshkhind, PUNE- 411 007. Retired from Food Technology Division, BARC, Mumbai. Ex–Professor(retired)-Department of Food engineering and technology, Institute of Chemical Technology, Mumbai-400 019.
The present study was undertaken to explore the rich and diverse coastal habitats of Konkan . Various samples like sand, clay, mud, sea-water, sea-weeds, alga, sea-shells, were collected from different sites on sea-coast of Maharashtra. (300Km). The microbial isolates were screened for the production of alkaline protease on sea-water based casein agar medium. The isolate RAT/SA/B24, producing alkaline protease was studied further. It was identified as Vibrio CJ11052 based on 16SrRNA sequencing. The alkaline protease produced was purified and partially characterized. The enzyme showed optimum activity at pH 9 , temperature 550C and was stable in saturating concentration of NaCl in the presence of calcium ions. Index Terms– Alkaline Protease, Halotolerant, Marine Habitats, Vibrio sp.
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