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International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
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Volume Issue
Volume-5, Issue-3, Spl. Iss-2  ( Sep, 2017 )
Paper Title
Study on Effect of Horseradish Peroidase for the Control of Hydrogen Peroxide Mediated Singal Generation through the Growth of Gold Nanoparticles
Author Name
Yufei Cheng, Cuong Cao, Chen Situ
Institute for Global Food Security School of Biological Sciences Queen’s University Belfast Northern Ireland, UK
In classical colorimetric immunoassays the signal for detection is generated by a bio-catalytic conversion of a molecule (antigen or antibody) by an enzyme which acts as an amplifier. However, a sensitive and reliable immunoassay requires high selectivity and affinity of antibodies to their corresponding antigens that forms the principle of bio-recognition. If the presence of analyte is at very low levels, immunoassay is unable to detect them and as such necessitates the use of more sensitive methods. Nanoparticles (e.g. gold or silver colloids etc) represent a new signal generation mechanism for biosensing giving the required improvements in signal production. A catalytic approach involving hydrogen peroxide and gold nanoparticles has recently been demonstrated and has the potential to be applied to a wide variety of food safety targets such as chemical and microbiological contaminants, veterinary drug residues, and protein biomarkers. Keywords - Immunoassay, Colorimetric, Bio-Recognition, Nanoparticle, Plasmonic, Contaminant.
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