DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-9085

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-5, Issue-2  ( Apr, 2017 )
Paper Title
Bioactivities of Protein Derived Peptides from Marine Crab Portunus Sanguinolentus
Author Name
Manjusha Sayd, Nafila Pp, Athulya M, Kunhi Aam
Affilition
Department of Biotechnology, SAFI Institute of Advanced Study, Vazhayoor, Malappuarm-673633
Pages
70-75
Abstract
Marine organisms are rich sources of structurally diverse bioactive compounds. Hence, a great interest has been developed nowadays to isolate bioactive compounds, which act as ACE inhibitors from marine resources because of their numerous health beneficial effects. Therefore, marine-derived bioactive peptides have a potential in use as functional ingredients in nutraceuticals and pharmaceuticals due to their effectiveness in both prevention and treatment of hypertension in addition to the nutritive value. Portunus sanguinolentus, three spotted crab is the commercially most important species of Portunus belongs to the family Portunidae (swimming crabs) which includes most of the economic crab species throughout the world. The objective of the present study is to determine the bioactive effect such as antioxidant, antimicrobial , anti cancer and antihypertensive activities of protein derived peptides from crab protein hydrolysate fractions of red three spotted crab shell hydrolysate. Insilico analysis results indicated that Crab derived peptides have bioactive effect. ACE inhibition studies of the crab protein derived peptides further showed a percentage inhibition was obtained in 50.1% and 57.2% for pepsin and trypsin digests, respectively. This crab protein derived peptides was on SDS PAGE analysis revealed the shorter peptide fragments. Antioxidants study revealed showed that the undigested protein sample (crude protein) exhibited a maximum antioxidant potential of 69%. The crude protein from crab gills also has shown a high potential of 66% antioxidant activity. The protease hydrolysis studies has been revealed that 8% pepsin digestion fraction of protein hydrolysate of P. sanguinolentus has exhibited maximum 50% of antioxidant potential. Marine-derived ACE inhibitors and their inhibitory properties, emphasizing their potential application can be used as nutraceuticals in future in preventing hypertension. Keywords - Bioactive peptide, ACE peptide, Crustacean, Crab, Protein derived peptide.
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