DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-9012

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
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Volume Issue
Issue
Volume-5, Issue-3, Spl. Iss-1  ( Aug, 2017 )
Paper Title
Development of a Flat Sheet Woven Fabric Membrane Fermenter for Xylanase Production by Thermomyces Lanuginosus
Author Name
V Thorulsley, S Rathilal, S Ramsuroop, Vl Pillay
Affilition
of Chemical Engineering, Institute of Water and Wastewater Treatment, Durban University of Technology, PO Box 1334, Durban 4000, South Africa Stellenbosch University, Department of Process Engineering, Private Bag X1, Matieland 7602, South Africa
Pages
58-63
Abstract
A submerged membrane fermenter system using a flat sheet woven fabric membrane to increase the rate of xylanase production, from the thermophilic fungal species Thermomyces lanuginous was developed and evaluated. During short run membrane fermenter experiments the percentage increase in enzyme activity between the batch operation and semi-continuous operation with beechwood xylan nutrient replenishment was 60.8%. The maximum volumetric productivity achieved with beechwood supplementation after 192 hours in semi-continuous operation was 2.1 times greater than that of batch operation which equates to an increase of 110% in productivity measured at its peak. During long run experimental periods, the increase in production of xylanase between the batch and the xylan medium membrane system was determined to be 74.3% whereas an overall average increase in productivity between the batch and xylan fed membrane system was 43.3%. Experiments where deionized water was alternated with beechwood xylan nutrient medium had no significant impact on the productivity or enzyme activity. The results are indicative of the potential viability of such a design, yielding the desired outcome of a membrane integrated system to significantly increase the production of enzymes during fermentation. Index Terms- Membrane fermenter, Thermomyces lanuginosus, woven fabric membrane, xylanase.
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