DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-4,Issue-3, Spl. Iss-1 ( Aug, 2016 ) | |||||||||
Paper Title |
A Bi-Functional Xylanase/ Xyloglucanase, GH5H, From Termite Metagenome Improves the Hydrolysis of Pretreated Biomass | |||||||||
Author Name |
Abhishek Bhattacharya, Mpho Mafa, Konanani Rashamuse, Brett I. Pletschke | |||||||||
Affilition |
Dept. of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa, 6140 Council for Scientific and Industrial Research, Pretoria, South Africa | |||||||||
Pages |
213-216 | |||||||||
Abstract |
Xyloglucan is a major component of the primary cell wall of higher plants and forms an intimate network with cellulose through hydrogen bonding and also with hemicellulosic components. In the present study, a bi-functional xylanase/xyloglucanase (GH5H) from a termite metagenome with high xylanase and xyloglucanase activities was used to replace Aspergillus niger xylanase in a previously established enzyme cocktail. The new combination was found to increase both glucose and xylose release from pre-treated biomass. Substrate competition assay indicated that xylanase and xyloglucanase activity is catalysed by the same active center. Keywords— Bi-functional enzyme, lignocellulose hydrolysis, termite GH5H. | |||||||||
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