DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-4,Issue-3, Spl. Iss-1 ( Aug, 2016 ) | |||||||||
Paper Title |
Evaluation of Brucella Antigens in Serological Diagnosis of Brucellosis | |||||||||
Author Name |
Aitbay K.Bulashev, Zhanbolat A.Suranshiev, Aibek.Kh.Zhumalin, Kanat A. Tursunov | |||||||||
Affilition |
Agricultural Biotechnology Research Institute, Astana, Kazakhstan | |||||||||
Pages |
138-140 | |||||||||
Abstract |
The diagnostic value of Brucella spp. outer membrane proteins (OMPs) in Enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of cattle brucellosis has been studied. It was established that B. abortus 19 and B. melitensis Rev-1 OMPs possess distinct antigenicity to antibodies of cows with positive results for brucellosis by Serum Agglutination Test and Complement Fixation Test. Brucella OMPs significantly exceeded the recombinant protein with a molecular weight of 26 kDa by their antigenicity. The results suggest that in the development of ELISA-tests for diagnosing brucellosis not single, but combination of several recombinant proteins should be used. Keywords- Antigenicity, Brucella spp., Enzyme-linked immunosorbent assay, Outer membrane proteins. | |||||||||
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