DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-4864

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-4,Issue-2, Spl. Iss-3  ( Jun, 2016 )
Paper Title
Identification Of A Golden Yellow Biocolorant From Microbacterium Pumilum NITD
Author Name
Susmita Das, Surojit Bera, Debjani Dutta
Affilition
Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713209, West Bengal, India
Pages
12-15
Abstract
Natural colorants like microbial pigment are gaining worldwide attention due to their strong colour and nutraceutical properties. The current study focuses on the extraction of pigment produced by a newly identified bacterium followed by characterization of the pigment by TLC and HPLC. The isolate was identified as Microbacterium pumilum. Growth pattern of the bacterium was studied in BHI medium at 30°C, initial pH 7.2 and 120 rpm. UV-Vis Spectrophotometry of the extracted pigment (in methanol) showed three distinct peaks at 428, 450 and 476nm which is an inevitably characteristic of a carotenoid i.e. zeaxanthin. Different solvent system was used to screen the favorable running phase for TLC with suitable resolution of the pigment. Both TLC and HPLC analysis showed the extracted pigment similar with standard zeaxanthin. Bacteria often produce a cocktail of polar and nonpolar carotenoids limiting their industrial applications. This study revealed that M. pumilum NITD produces zeaxanthin as a major carotenoid in its stationary phase and could be used as a potent zeaxanthin producer for future applications. Index Terms— Carotenoids, Zeaxanthin, Microbacterium pumilum, HPLC
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