DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-4,Issue-1, Spl. Iss-3 ( Mar, 2016 ) | |||||||||
Paper Title |
One-Step DNA Extraction For Detection Of Listeria Monocytogenes | |||||||||
Author Name |
Sirirat Wachiralurpan, Thayat Sriyapai, Supatra Areekit, Thongchai Kaewphinit, Pichapack Sriyapai, Somchai Santiwatanakul, Kosum Chansiri | |||||||||
Affilition |
Faculty of Medicine, Srinakharinwirot University Thailand, Faculty of Environmental culture and Ecotourism, Srinakharinwirot University Thailand, Innovation Learning Center, Srinakharinwirot University Thailand, Faculty of Science, Srinakharinwirot University Thailand | |||||||||
Pages |
144-145 | |||||||||
Abstract |
Conventional routine detection of Listeria monocytogenes in food products requires at least 7 days. Thus, the rapid technology for detection of L. monocytogenes is of the utmost importance and urgent necessity. This study aims to develop the rapid DNA extraction for detection of L. monocytogenes by using PCR assay. After DNA extraction of L. monocytogenes, three methods including the deionized nanopure water and 0.001% SDS solution with or without boil methods cloud be used for the one-step DNA extraction. Keywords- one-step DNA extraction, Listeria monocytogenes, polymerase chain reaction. | |||||||||
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