DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-4,Issue-1, Spl. Iss-2 ( Feb, 2016 ) | |||||||||
Paper Title |
Optimization Of Bovine Serum Albumin Fibrillation By Congored Spectrophotometry For Using As A New Bio-Nanomaterial | |||||||||
Author Name |
Amir Arasteh, Marzieh Rashvand, Azadeh Ebrahim Habibi, Mehran Habibi Rezaei, Ali Akbar Moosavi Movahedi | |||||||||
Affilition |
Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran Department of microbiology, falavarjan Branch, Islamic Azad University, falavarjan-Esfahan, Iran Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran Protein Technology Research Lab (PBRL), School of Biology, College of Science, University of Tehran, Tehran, Iran Institute of Biochemistry and Biophysics, Univers | |||||||||
Pages |
59-65 | |||||||||
Abstract |
Aim and Background: Amyloids are fibrilar protein structures produced from aggregation of proteins and peptide together. In recent years, amyloid fibrils are being considered as new experimental nanomaterials and from this perspective, increased levels of amyloid proteins can be desirable. In this study, the fibrillation of bovine serum albumin (BSA) as a model protein is optimized. Materials and Methods: Different concentrations of protein were subjected to different pH and temperature and amount of amyloid fibrils were detected by spectrophotometric Congored binding assay. This dye can integrate to fibrous amyloid structures and change the absorbance and λmax of fibrillated protein comparison to native form. Results: In this research, effect of four variables include temperature, pH, time of incubation and protein concentration was investigated on fibrillogenesis and results were confirmed by Congored spectrophotometric method as λmax and absorbance in λmax (Aλmax) accompanying with transmission electron microscopy. The optimum condition for fibrillogenesis was specified in 5 mg.ml-1 of protein and buffer pH of 3 after 72 hour incubation in 70ºC. Conclusion: simple Congored spectrophotometric method could be used as primary test for evaluating protein nanobiofibrils and absorbance in λmax is introduced as a valid indicator in this way. Keywords— Bovine Serum Albumin, Amyloid, Spectrophotometry, Congored. | |||||||||
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