DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-8,Issue-4 ( Oct, 2020 ) | |||||||||
Paper Title |
Menthol based Hydrophobic Deep Eutectic Solvent for Extraction and Purification of Ergosterol using Response Surface Methodology | |||||||||
Author Name |
Lalit Khare, Tanvi Karve, Ratnesh Jain, Prajakta Dandekar | |||||||||
Affilition |
Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, N.P. Marg, Matunga, Mumbai 400019, Maharashtra, India Department of Chemical Engineering, Institute of Chemical Technology, N.P. Marg, Matunga, Mumbai 400019, Maharashtra, India | |||||||||
Pages |
32-38 | |||||||||
Abstract |
Vitamin D deficiency has been linked to various diseases, but could be rectified via fortified food stuffs or sup- plementation. In this study 39 different hydrophobic deep eutectic solvents were evaluated for green extraction of ergosterol from mushroom. Extraction parameters (e.g. time, solvent volume) were optimized using response surface methodology (RSM) and a maximum extraction yield of 6995.00 μg ergosterol/g dry weight mushroom was attained with menthol: pyruvic acid. The extracted ergosterol was purified using a novel methodology and the extraction solvent was reused for six cycles, while retaining extraction efficiency (up to 28%). The ergosterol was exposed to ultra-violet radiation for conversion to ergocalciferol (vitamin D2) resulting in a yield of ergocal- ciferol that was equivalent to 2142.01 μg/g dry weight mushroom. Keywords - Vitamin D2, Ergosterol, Hydrophobic Deep Eutectic Solvent, Menthol Pyruvic Acid, Extraction | |||||||||
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