DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-16667

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-7,Issue-4  ( Oct, 2019 )
Paper Title
Semi-Quantitative Analysis of Nitric Oxide Production in Three-dimensional Glioblastoma Multiforme Models in Vitro
Author Name
Elena Afrimzon, Yana Shafran, Naomi Zurgil, Maria Sobolev, Mordechai Deutsch
Affilition
The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Bar-Ilan University, Ramat Gan, 52900, Israel
Pages
71-76
Abstract
Glioblastoma Multiforme (GBM) remains an incurable brain disease. One of the most important signaling molecules in the brain, nitric oxide (NO) mediates important inter- and intracellular interaction in astrocytes and neurons. In the presented study, the three-dimensional glioblastoma multicellular models were established from A-172 cells and grown in vitro in a picowell array. "Intra-spheroid" NO content and its association with mitochondrial function were studied at a resolution of single 3D object. The association between "intra-spheroid" levels of nitric oxide and mitochondrial status is revealed in real-time measurements and may serve as a significant target for tumor cure. Keywords - Glioblastoma Multiforme, Nitric Oxide, Mitochondrial Membrane Potential, Three-Dimensional Multicellular Models in Vitro
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