DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-7,Issue-4 ( Oct, 2019 ) | |||||||||
Paper Title |
Heavy Metal Accumulation by Saccharomyces Cerevisiae Cells Expressing Cup1cystein-Rich Olygopeptides Targeted to The Inner Face of The Plasma Membrane | |||||||||
Author Name |
Ileana Cornelia Farcasanu, Aurora Daniela Neagoe, Lavinia Liliana Ruta | |||||||||
Affilition |
Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania. Faculty of Biology, University of Bucharest, Spl. Independentei, 91-95, Bucharest, Romania | |||||||||
Pages |
39-44 | |||||||||
Abstract |
Cup1 is the Saccharomyces cervisiaemetallothionein responsible for buffering the surplus copper taken up by yeast cells. In this study we obtained S. cerevisiae cells armed with three metal-binding oligopeptides derived from Cup1 sequence targeted to the inner face of the plasma membrane. The three metal binding oligoppetidescorrespon to three cystein-rich metal-binding sequences (MeBSeq1-3) from Cup1 primary stucture. We describe the constructionof S. cerevisiaestrains expressing MeBSeq1-3 fused to the carboxy-terminus of amyristoylated green fluorescent protein (myrGFP). These strains were investigated in terms of tolerance to heavy metals exposure and heavy metal accumulation. Keywords - Heavy Metal, Saccharomyces Cerevisiae, Cup1, Metal-Binding Sequence | |||||||||
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