DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-16436

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-7,Issue-4  ( Oct, 2019 )
Paper Title
Use of Brucella Recombinant Proteins in Differentiating Infected from Vaccinated Cattle
Author Name
Aitbay Bulashev, Orken Akibekov, Vladimir Kiyan, Kanat Tursunov, Gulgaisha Mukhitden, Saule Eskendirova
Affilition
S. Seifullin Kazakh Agrotechnical University, Republic of Kazakhstan National Center for Biotechnology, Republic of Kazakhstan
Pages
23-26
Abstract
In this study the reactivity of the recombinant outer membrane (rOmp19, rOmp25, rOmp31) and periplasmic proteins (rBP26, rSOD) in serum samples, taken from cows that is kept in Brucella-contaminated and brucellosis free farms where B. abortus S19 vaccine had been used for immunization, was tested. The results showed that vaccinated cattle from brucellosis free herd could develop an antibody response to Brucella rOmps and rBP26. It was found that the rSOD provided the most reliable differentiation infected from vaccinated animals. Keywords - Brucellosis, Diagnosis, Conventional Serological Tests, Enzyme Linked Immunosorbent Assay.
  View Paper