DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-1620

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-3, Issue-1  ( Jan, 2015 )
Paper Title
Microfluidic Single Cell Bioanalysis To Measure Drug Uptake On Single Multidrug Resistant Cancer Cell As Enhanced By Ginsenoside Rg3
Author Name
Yuchun Chen, Iryna Kolesnyk, Paul Ch Li, Patrick y. K. Yue, Ricky N.S. Wong
Affilition
Department of Chemistry, Department of Molecular Biology and Biochemistry, Simon Fraser University, Department of Biology, Baptist University
Pages
5-7
Abstract
This paper is to demonstrate that the microfluidic-based single cell chip can be applicable to study micro-gram level amount of ginsenosides on daunorubicin uptake in multidrug resistant (MDR) leukemia cells. Pure ginseng compounds are very hard to obtain and can only be obtained at a trace amount (mini grams level). This small amount sometimes is not enough for traditional methods to evaluate the MDR effect of these compounds. Here, the microfluidic single cell analysis method was used to evaluate the MDR effect of several ginsenosides. In this paper, we tested two ginsenoside compounds: 20(S)-Ginsenoside Rg3 (Rg3-S) and 20(R)-Ginsenoside Rg3 (Rg3-R)and found that Rg3-S provided a stronger effect on enhancing the intracellular accumulation of anticancer drug in the single MDR cancer cells.. Keywords- Cancer cell, Fluorescence, Ginsenosides, Microfluidics.
  View Paper