DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-7,Issue-1 ( Jan, 2019 ) | |||||||||
Paper Title |
In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders | |||||||||
Author Name |
Boyka Andonova-Lilova, Radostina Alexandrova, Kostadinka Sezanova, Rumiana Gergulova, Diana Rabadjieva | |||||||||
Affilition |
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 11, 1113 Sofia, Bulgaria | |||||||||
Pages |
1-6 | |||||||||
Abstract |
Zinc modified (Zn/(Zn+Ca) = 0, 0.03, 0.13) calcium phosphate fine powders were synthesized by biomimetic approach and subsequent high-temperature treatment. Bi-phase mixture of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) was prepared at non modified sample and monophase Zn-β-TCP at Zn modified samples. The in vitro biological behavior of the prepared powders was studied using human Lep-3 and bovine MDBK cells. The effect of the materials on cell viability and proliferation was evaluated by three methods with different molecular / cellular targets and mechanisms of action - MTT test, neutral red uptake assay and crystal violet staining. The results obtained revealed high viability of both Lep-3 and MDBK cells cultured for 72h and 96h in the presence of the materials examined applied as 100% material-modified cell culture medium in all three cases. The dilution of material-modified cell culture medium with nonmodified culture medium leads to increasing of cell viability. | |||||||||
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