DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-6, Issue-3, Spl. Iss-2 ( Sep, 2018 ) | |||||||||
Paper Title |
The Prevalence of Metallo-β-Lactamase Genes in Imipenem and Meropenem Resistant Pseudomonas aeruginosa Isolated from Patients in El-Obied Hospitals – Sudan | |||||||||
Author Name |
Wafaa Mohammed Adam Abdulrahaman, Eldaw Breima Suliman Mohamed, Mustafa Zaki Ibrahim Ali | |||||||||
Affilition |
Department of Laboratory, College of Medicine, Batterjee Medical College, KSA, Department of Microbiology, Faculty of Medicine and Health Sciences, University of Kordofan, Sudan, Department of Microbiology and Parasitology, Soba University Hospital | |||||||||
Pages |
9-12 | |||||||||
Abstract |
Acquired Metallo-β-lactamases (MBLs) are emerging worldwide as powerful resistance determinants. So far, six MBL enzyme types have been described in clinical isolates of P. aeruginosa-VIM, IMP, SPM, GIM, NDM and AIM type.This study aimed to determine the prevalence of Metallo-β-Lactamase genes in imipenem and meropenem-resistant Pseudomonas aeruginosa isolated from Elobied Hospitals. All collected specimens (721) were cultured and identified using API 12A/12E. Hundred (100) isolated Pseudomonas aeruginosa were subjected to antimicrobial susceptibility testing (Kirby Bauer), for selected imipenem and meropenem. PCR was performed for detection of blaVIM-1, NDM-1and VIM like genes in imipenem and meropenem resistant P. aeruginosa strains. Nineteen (19%) and 14(14%) isolates were found resistant to imipenem and meropenem respectively, 1/19(5.26 %) of isolated resistant P. aeruginosa carry the NDM-1 gene, and 1/14 (7.1 %) carried the VIM gene which encoding resistant to imipenem and meropenem respectively, while no detection to blaVIM-1gene in our study. The study concluded that evidence of the presence of the NDM-1 and VIM genes, while none of the isolated P. aeruginosa strains carry blaVIM-1. Further studies and a regular monitoring system for early detection of MBL-producing organisms are recommended. Keywords - MBLs, P. aeruginosa, NDM-1, VIM-1, VIM, imipenem and meropenem. | |||||||||
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