DOIONLINE

DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-13120

Publish In
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT
Journal Home
Volume Issue
Issue
Volume-6, Issue-3  ( Jul, 2018 )
Paper Title
Antioxidant Activity Fraction of Kaffir Lime Leaves (Citrus Hystrix Dc.) By 1,1-Diphenyl-2-Picrylhydrazil (DPPH) Radical Scavenging Method
Author Name
Nur Chaerun Nisa, Aktsar Roskiana Ahmad, Rezki Amriati Syarif, Andi Amaliah Dahlia
Affilition
Faculty of Pharmacy, Universitas Muslim Indonesia, Jl. Urip Sumoharjo KM 5 Makassar Indonesia
Pages
11-15
Abstract
The sample used in this study is the leaves of Citrus hystrix DC. belong to the family Rutaceae. This research aimed to determine the antioxidant activity of Citrus hystrix DC. leave fraction using 1,1-diphenyl-2-picrylhydrazil (DPPH) free radical scavenging method. Citrus hystrix DC. leaf extract was extracted by maceration using ethanol 96% and obtained ethanol extract yield value of 5,044% and subsequently fractionated by Vacuum Liquid Chromatography (VLC) with the mobile phase is n-hexane:ethyl acetate and ethyl acetate:methanol. The stationary phase used silica gel F254. The number of fractions obtained are 4 fractions. The results showed that the Citrus hystrix DC. leave fraction has antioxidant activity. The value of IC50 fraction 1 is 275,814 μg/mL, fraction 2 is 200,297 μg/mL, fraction 3 is 242,247 μg/mL and fraction 4 is 121,831 μg/mL. The 4thfraction of the Citrus hystrix DC. leave has higher antioxidant than the other fractions. Index terms - Antioxidant, Citrus hystrix DC., kaffir lime leaves, 1,1-diphenyl-2-picrylhydrazil (DPPH), Vacuum Liquid Chromatography (VLC).
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