DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-6, Issue-2 ( Apr, 2018 ) | |||||||||
Paper Title |
Resistin Stimulates the Expression of Suppressor of Cytokine Signaling 3 Gene in 3T3-L1 Adipocytes through Multiple Kinase Pathway | |||||||||
Author Name |
Yen-Yue Lin, Chi-Peng Wu, Yi-Wei Tsuei, Yow-Chii Kuo, Chi-Wei Liu, An-Ci Siao, Yung-Hsi Kao | |||||||||
Affilition |
Department of Life Sciences, National Central University, Taoyuan City, Taiwan Taoyuan Armed Forces General Hospital, Taoyuan City, Taiwan Landseed General Hospital, Taoyuan City, Taiwan Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan City, Taiwan | |||||||||
Pages |
16-21 | |||||||||
Abstract |
Resistin (Rstn) and suppressor of cytokine signaling (SOCS)-3 have been reported to regulate fat cell activity and insulin resistance, respectively. Using 3T3-L1 adipocytes and the method of real-time PCR, we investigated the pathways involved in Rstn-stimulated SOCS-3 gene expression. The up-regulation of SOCS-3 mRNA expression induced by Rstn was observed. Treatment with an inhibitor of Janus kinase (JAK)-2, such as AG490, blocked Rstn-increased levels of SOCS-3 mRNA. Moreover, pretreatment with specific inhibitors of either ERK1/2 (U0126), p38 MAPK (SB203580), JNK (SP600125), or phosphatidylinositol 3-kinase/AKT (LY294002 and wortmannin) prevented Rstn-increased levels of SOCS-3 mRNA. These results imply that JAK2, PI3K, ERK1/2, p38, and JNK MAPKs are necessary for the Rstn stimulation of SOCS-3 gene expression. Keywords - Resistin, Adipocyte, SOCS-3, Kinase. | |||||||||
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