DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-6, Issue-2 ( Apr, 2018 ) | |||||||||
Paper Title |
Isolation, Characterization and Identification of DNA’S Lactic Acid Bacteria (LAB) of Dadih Lintau (Tanah Datar District) which is Antibiotic Resistance | |||||||||
Author Name |
Endang Purwati, James Hellyward, Yuherman, Hendri Purwanto, Puji Hartini R | |||||||||
Affilition |
Professor of Laboratory Biotechnology/ Technology of Product Husbandry Faculty of Animal Husbandry University of Andalas Padang West Sumatera Indonesia Professor of Laboratory Biotechnology/ Technology of Product Husbandry Faculty of Animal Husbandry University of Andalas Padang West Sumatera Indonesia Doctor of Laboratory Biotechnology/ Technology of Product Husbandry Faculty of Animal Husbandry University of Andalas Padang West Sumatera Indonesia Master of Laboratory Biotechnology/ Techn | |||||||||
Pages |
28-31 | |||||||||
Abstract |
Research about isolation, characterization, and identification of DNA’s lactic acid bacteria of dadih Lintau Tanah Datar District which is antibiotic resistant has been done. Eleven isolates were isolated; U4, U5, U6, U7, U8, U9, U10, U11, U12, U13 and U14; characteristic of isolates are broken white, round shape, convex, edge slick and Gram positive bacteria. Nine (9) isolates are rod cell, 2 others are cocci. isolates were characterized based on antibiotic resistance. Ampicillin 10 μg (AM 10), Diclixacillin 1 μg (DCX1), Gentamicin 10 μg (CN 10), Cofeperazone 75μg (CFP 75), Carbenicillin 100μg (CB 100) have been used as antibiotics test. The result showed that six isolates resistance to all of antibiotics; U4, U5, U6, U7, U8 and U11. The result of DNA identification by primer 27F-1429R, showed that PCR product about 1500 base pair. BLAST analisys result from all dadih Lintau isolate include the lactic acid bacteria group, match 97% - 100% with Lactobacillus plantarum. Keyword - Antibiotic, Buffalo Milk, Dadih, Fermented, Lactic Acid Bacteria | |||||||||
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