DOIONLINE 
DOIONLINE Publish In |
International Journal of Advances in Science, Engineering and Technology(IJASEAT)-IJASEAT |
 Journal Home Volume Issue |
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Issue |
Volume-6, Issue-2 ( Apr, 2018 ) | |||||||||
Paper Title |
Quantitative Protein Expression of Malaysian Phaleriamacrocarpa | |||||||||
Author Name |
Noraslinda Muhamad Bunnori, Nurul Ashima Hamdan, Muhamad Shafiq Abu Hassan, Aisyah Mohamed Rehan, Khairul Bariyyah Abd Halim, Normah Haron | |||||||||
Affilition |
Kulliyyah of Science, International Islamic University Malaysia, Bandar InderaMahkota, 25200 Kuantan, Malaysia | |||||||||
Pages |
18-22 | |||||||||
Abstract |
Herbal plants are the best alternative for synthetic medicines which are costly and proved to be double-edged source. P. macrocarpa is the traditional herb compatible in curing diseases like diabetes, inflammation, and even cancer. People have gained lots of benefits from most parts of the plant with largest usage of seeds. This study aimed to analyze protein expressions in three major parts of the plant; leaf, seed and fruit by using SDS PAGE and high-throughput twodimensional gel electrophoresis as proteomics approached. There were 42 spots detected in a pH range of 3-10 with 3 spots have been analyzed by MALDI-TOF mass spectrometry. Most identified proteins were expressed in seeds with highest intensity of bands and spots. The identified spots are homologs to putative clathrin assembly protein and ferredoxin-NADP+ reductase which are characterized as ‘housekeeping agent’ in plant system. Keywords- Protein expression, phaleriamacrocarpa, 2D-PAGE, clathrin, ferredoxin | |||||||||
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